The wheat Lr34 multipathogen resistance gene confers resistance to anthracnose and rust in sorghum
Schnippenkoetter W, Lo C, Liu G, Dibley K, Chan WL, White J, Milne R, Zwart A, Kwong E, Keller B, Godwin I, Krattinger SG and Lagudah ES (2017) The wheat Lr34 multi-pathogen resistance gene confers resistance to anthracnose and rust in sorghum. Plant Biotechnology Journal 15, 1387-1396.
The ability of the wheat Lr34 multipathogen resistance gene (Lr34res) to function across a wide taxonomic boundary was investigated in transgenic Sorghum bicolor. Increased resistance to sorghum rust and anthracnose disease symptoms following infection with the biotrophic pathogen Puccinia purpurea and the hemibiotroph Colletotrichum sublineolum, respectively, occurred in transgenic plants expressing the Lr34res ABC transporter. Transgenic sorghum lines that highly expressed the wheat Lr34res gene exhibited immunity to sorghum rust compared to the low‐expressing single copy Lr34res
genotype that conferred partial resistance. Pathogen‐induced
pigmentation mediated by flavonoid phytoalexins was evident on
transgenic sorghum leaves following P. purpurea infection within 24–72 h, which paralleled Lr34res gene expression. Elevated expression of flavone synthase II, flavanone 4‐reductase and dihydroflavonol reductase genes which control the biosynthesis of flavonoid phytoalexins characterized the highly expressing Lr34res transgenic lines 24‐h post‐inoculation with P. purpurea. Metabolite analysis of mesocotyls infected with C. sublineolum showed increased levels of 3‐deoxyanthocyanidin metabolites were associated with Lr34res expression, concomitant with reduced symptoms of anthracnose.